Journal: International Journal of Molecular Sciences

Article Title: Binding of Organometallic Ruthenium Anticancer Complexes to DNA: Thermodynamic Base and Sequence Selectivity

doi: 10.3390/ijms19072137

Figure Lengend Snippet: High performance liquid chromatography (HPLC) chromatograms with ultraviolet (UV) detection at 260 nm for reactions of ruthenium complexes with single strand I . ( A ) Complex 1 ( i ), 2 ( ii ), 3 ( iii ), or 4 ( iv ) with single strand I (0.1 mM, Ru/ I = 1.0) in 50 mM triethylammonium acetate buffer (TEAA) (pH 7) at 310 K for 24 h; ( B ) complex 1 with I (0.1 mM, Ru/ I = 1.0) in 50 mM TEAA (pH 7) and 5 mM NaCl at 310 K for different times. Peak assignments: a, unruthenated strand I ; b, c, d, and e, mono-ruthenated I ; f, g, and h, di-ruthenated I . For mass spectra, see .

Article Snippet: Triethylammonium acetate buffer (TEAA, 1 M) was purchased from AppliChem (Darmstadt, Germany), and acetonitrile (HPLC grade) from Tedia (Fairfield, OH, USA).

Techniques: High Performance Liquid Chromatography

Journal: International Journal of Molecular Sciences

Article Title: Binding of Organometallic Ruthenium Anticancer Complexes to DNA: Thermodynamic Base and Sequence Selectivity

doi: 10.3390/ijms19072137

Figure Lengend Snippet: ( A – D ) HPLC chromatograms for reaction mixtures of complex 1 ( A ), 2 ( B ), 3 ( C ), or 4 ( D ) with single strand I (0.1 mM) at various molar ratios in 50 mM TEAA at 310 K for 24 h. Peak assignments: a, unruthenated strand I ; b, mono-ruthenated I ; c, di-ruthenated I . ( E ) A direct plot (dots) of moles of ODN-bound 1 , 2 , 3 , or 4 ([Ru] B ) as a function of the concentration of free ruthenium complexes ([Ru] F ). Computer-fitting (lines) of the experimental data to the ligand–receptor binding Equation (8) (see details in ) gave rise to the equilibrium constants for the two-site binding reactions listed in .

Article Snippet: Triethylammonium acetate buffer (TEAA, 1 M) was purchased from AppliChem (Darmstadt, Germany), and acetonitrile (HPLC grade) from Tedia (Fairfield, OH, USA).

Techniques: Concentration Assay, Binding Assay

Journal: International Journal of Molecular Sciences

Article Title: Binding of Organometallic Ruthenium Anticancer Complexes to DNA: Thermodynamic Base and Sequence Selectivity

doi: 10.3390/ijms19072137

Figure Lengend Snippet: Total ion count (TIC) chromatograms for reactions of complex 1 ( i ), 2 ( ii ), 3 ( iii ), or 4 ( iv ) with single strand II (0.1 mM) at the molar ratio of ( A ) Ru/ II = 1.0 or ( B ) Ru/ II = 3.0 in 50 mM TEAA (pH 7) at 310 K for 24 h. Peak assignments: a, unruthenated strand II ; b, d, f, and h, mono-ruthenated II ; c, e, g, and k, di-ruthenated II ; m, n, and p, tri-ruthenated II . Notably the fraction c in ( B ) contains a little tri-ruthenated II by 1 and fractions n and p contains a small amount of tetra-ruthenated II by 3 or 4 as shown in .

Article Snippet: Triethylammonium acetate buffer (TEAA, 1 M) was purchased from AppliChem (Darmstadt, Germany), and acetonitrile (HPLC grade) from Tedia (Fairfield, OH, USA).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Binding of Organometallic Ruthenium Anticancer Complexes to DNA: Thermodynamic Base and Sequence Selectivity

doi: 10.3390/ijms19072137

Figure Lengend Snippet: ( A ) HPLC chromatograms with ultraviolet (UV) detection at 260 nm for the reaction mixture of duplex III with complex 1 ( i ) or complex 4 ( ii ) (Ru/ III = 1.0) in 50 mM TEAA buffer (pH 7) and 100 mM NaClO 4 incubated at 310 K for 48 h; ( B ) mass spectra for HPLC fractions shown in ( A ); ( C ) circular dichroism (CD) spectra of free duplex III (black) and the reaction mixtures of duplex III with complex 1 (red), 2 (green), 4 (blue) and cisplatin (magenta) incubated at 310 K for 24 h at a molar ratio of Ru/ III or Pt/ III = 6.0.

Article Snippet: Triethylammonium acetate buffer (TEAA, 1 M) was purchased from AppliChem (Darmstadt, Germany), and acetonitrile (HPLC grade) from Tedia (Fairfield, OH, USA).

Techniques: Incubation, Circular Dichroism